Application
Western blot
Sample
Mouse Cell lysate - whole cell (Intestine tissue)
Gel Running Conditions
Reduced Denaturing (7.5% gel SDS)
Loading amount
50 µg
Specification
Intestine tissue
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Liwei Xie
Verified customer
投稿 Jul 05 2012