Application
ChIP
Sample
Rat Cell lysate - nuclear (rat PC12 cell)
Specification
rat PC12 cell
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde (final conc: 1% )
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde (final conc: 1% )
Detection step
Semiquantitative PCR
Positive control
In the DNA electrophoretogram, lane 4 is another anti-SP1 Ab (millipore, Cat. 17-601) that reacts with rat.
Negative control
In the DNA electrophoretogram, lane2 is anti-rabbit IgG.
Other product details
Dilution
1/10
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: WCE buffer
Additional data
Additional Notes
1. Rat SPHK1 primer design was performed according to this paper "Transcription factor speci?city protein 1 (Sp1) is the main regulator of nerve growth factor-induced sphingosine kinase 1 gene expression of the rat pheochromocytoma cell line, PC12"(2005).
2. PCR condition were 94? for 30 s,54? for 30 s, followed by 72? for 30 s. The number of cycles was 30.
Positive control: In the DNA electrophoretogram, lane 4 is another anti-SP1 Ab (millipore, Cat. 17-601) that reacts with rat.
Negative control: In the DNA electrophoretogram, lane 2 is anti-rabbit IgG.
2. PCR condition were 94? for 30 s,54? for 30 s, followed by 72? for 30 s. The number of cycles was 30.
Positive control: In the DNA electrophoretogram, lane 4 is another anti-SP1 Ab (millipore, Cat. 17-601) that reacts with rat.
Negative control: In the DNA electrophoretogram, lane 2 is anti-rabbit IgG.
Abcam response
Thank you so much for testing this antibody in rat. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 02 2012