Application
Western blot
Sample
Cow Cell lysate - whole cell (chondrocytes lysate in RIPA buffer)
Loading amount
20 µg
Specification
chondrocytes lysate in RIPA buffer
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 37°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/30000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 55 kDa Non-specific: 75 kDa
Additional data
Additional Notes
1. primary chondrocytes
2. passaged chondrocytes
2. passaged chondrocytes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Nazish Ahmed
Verified customer
投稿 Dec 02 2011