Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Concentration
1 µg/ml
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 150, 65, 68, 50, 40, 37 kDa
Positive control
rat brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 03 2016