Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (human normal keratinocytes and SCC cancer cell lin)
Specification
human normal keratinocytes and SCC cancer cell lin
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tris, NaCl, 0.2% Tween, 2.5% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 680-conjugated Donkey anti Mouse (Jack
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 790
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 790
Dilution
1/20000
Detection
Detection method
fluorescence
Bands
Specific: none kDa Non-specific: 85 kDa
Exposure
30 second(s)
Positive control
human keratinocytes
Additional data
Abcam response
Thank you for testing this antibody in on an untested species (Human). We appreciate your effort and feedback but are sorry to hear that the antibody did not work in WB on Human cell lysates. As stated on the datasheet, we do not recommend this antibody for use on Human samples. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 13 2014