Application
IHC - Wholemount
Sample
Mouse Tissue (Brain)
Specification
Brain
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey Anti-Rabbit Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Tissue was perfusion fixed with 4% paraformaldehyde in sodium cacodylate buffer (pH: 7.4 15 mins.) then immersion fixed overnight at 4 degrees Celsius. Brains were then sectioned using a Leica vibratome at a thickness of 100 microns. Sox2 (green) Hoescht (blue) GFAP (red).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Gabriel Luna
Verified customer
投稿 Jan 04 2018