Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Nasopharynx)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6.0; 20min; 100*C
Permeabilization
No
Specification
Nasopharynx
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako Diluent, Background Reducers
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Leica Refine Polymer Detection, anti-Mouse/Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Full procedure automated on Leica Bond RX.
Various pretreatment regimens tested (none, proteinase K, citrate buffer, EDTA buffer). Citrate gave best signal to background reaction.
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投稿 Dec 21 2016