Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kiney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kiney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
16 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: ~55 kDa Non-specific: multiple kDa
Positive control
Mouse brain Lysate
Additional data
Additional Notes
Goat anti rabbit secondary antibody was used
Abcam response
Thank you for sending your testing results in COS7 cells. Unfortunately, it looks like the antibody did not recognise the protein specifically in this sample type. We encourage further Abtrial testing, if of interest.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 02 2016