Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E10.5 mouse embryo)
Permeabilization
Yes - PBS/0.1% Triton X-100
Specification
E10.5 mouse embryo
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: R.T°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Tested both with and without antigen retrieval. Antigen retrieval was performed by boiling the slides in 10mM Sodium Citrate (pH 6) for 5 min. Any obvious signal in the images is non-specific corresponding to autofluorescence. Cell nuclei were stained with DAPI (blue).
Abcam response
Thank you for sharing this data with us. ab109290 has not previously been tested in IHC-Fr. Based on this abreview, ab109290 does not work in IHC-Fr, under these experimental conditions. Abcam would welcome feedback from other researchers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Anestis Tsakiridis
Verified customer
投稿 Oct 30 2015