Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (differentiating PC12 cells)
Specification
differentiating PC12 cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Concentration
2 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Differentiated PC12 cells were counterstained with the nuclear TOPRO-3 dye. This antibody works equally well for immunoblot analysis.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Anne Chiaramello
Verified customer
投稿 Apr 18 2008