Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
Sample
Rat Tissue sections (sciatic nerve (injured with lymphatic cells penetr)
Specification
sciatic nerve (injured with lymphatic cells penetr
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red
Dilution
1/200
Additional data
Additional Notes
rat sciatic nerve (would introduced and 9 days after tissue regeneration) was formalin fixed paraffin embedded. The positive staining is detected in lymphatic tissue (lymphocytes) around nerve tissue as indicated by red fluorescence (Texas Red).
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投稿 Aug 28 2014