Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte-derived macrophage)
Loading amount
30 µg
Specification
monocyte-derived macrophage
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Goat IgG (whole molecule)–Peroxidase antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 45 kDa
Additional data
Additional Notes
Lane on the Western blot indicates 30 microgram of whole-cell lysates from untreated human monocyte-derived macrophages
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 17 2013