Anti-SNX30 抗体 (ab121600)
Key features and details
- Rabbit polyclonal to SNX30
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SNX30 antibody -
製品の詳細
Rabbit polyclonal to SNX30 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: VGGDSTPSPD LLMARSFGDK DLILPNGGTP AGTSSPASSS SLLNRLQLDD DIDGETRDLF VIVDDPKKHV CTMETY, corresponding to amino acids 33-108 of Human SNX30.
-
ポジティブ・コントロール
- Human duodenum tissue and RT4, U 251 MG, Human Liver and Tonsil lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121600の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
特記事項 |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
-
機能
May be involved in several stages of intracellular trafficking. -
配列類似性
Belongs to the sorting nexin family.
Contains 1 PX (phox homology) domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 401548 Human
- SwissProt: Q5VWJ9 Human
- Unigene: 522350 Human
-
別名
- snx30 antibody
- SNX30_HUMAN antibody
- Sorting nexin-30 antibody
画像
-
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab121600 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
ab121600 at 1/125 dilution staining SNX30 in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in glandular cells. -
All lanes : Anti-SNX30 antibody (ab121600) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab121600 は 2 報の論文で使用されています。
- Antón Z et al. A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly. J Cell Sci 133:N/A (2020). PubMed: 32513819
- Simonetti B et al. Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR. J Cell Biol 216:3695-3712 (2017). PubMed: 28935633