Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Loading amount
25 µg
Specification
HEK293T cells
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C
Secondary antibody
Name
Non-Abcam antibody was used: Li-Cor anti-Mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
Odyssey Infrared Imaging System
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~60 kDa
Positive control
This sample
Additional data
Additional Notes
TritonX100 soluble proteins from a HEK293T cell lysate were separated on a 4-12% SDS-PAGE gel and transferred to PVDF. SNX27 was detected using a Infrared imagining system. Scan intensity was set to 7.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 24 2013