Anti-SNAP23 抗体 (ab3340)
Key features and details
- Rabbit polyclonal to SNAP23
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SNAP23 antibody
SNAP23 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SNAP23 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Mouse SNAP23 aa 193-210.
Sequence:NKNRIDIANTRAKKLIDS
(Peptide available asab4956) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3340の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (6) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. 23kDa band represents SNAP 23 from rat brain protein extract.
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ICC/IF |
Use a concentration of 2 µg/ml.
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IP | (1) |
Use at an assay dependent concentration.
Used at a concentration of 2 ug/ml for 1 hr (see Abreview). |
IHC-P |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. 23kDa band represents SNAP 23 from rat brain protein extract. |
ICC/IF
Use a concentration of 2 µg/ml. |
IP
Use at an assay dependent concentration. Used at a concentration of 2 ug/ml for 1 hr (see Abreview). |
IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion. -
組織特異性
Ubiquitous. Highest levels where found in placenta. -
配列類似性
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
細胞内局在
Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8773 Human
- Entrez Gene: 20619 Mouse
- Entrez Gene: 64630 Rat
- Omim: 602534 Human
- SwissProt: O00161 Human
- SwissProt: O09044 Mouse
- SwissProt: O70377 Rat
- Unigene: 511149 Human
see all -
別名
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
see all
画像
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All lanes : Anti-SNAP23 antibody (ab3340) at 1 µg/ml
Lane 1 : Untransfected U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates
Lane 2 : Non-specific scrambled siRNA transfected U-87 MG whole cell lysates
Lane 3 : SNAP23 KO U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/mlDecrease in signal upon siRNA mediated knock down confirms that antibody is specific to SNAP23.
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Immunofluorescence analysis of SNAP-23 was performed using 90% confluent log phase U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells with ab3340. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3340 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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ab3340 (1ug/ml) staining SNAP23 in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cellular membrane compartments of the lymphatic nodules.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
All lanes : Anti-SNAP23 antibody (ab3340) at 2 µg/ml
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg/ml per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.4 µg/ml -
Anti-SNAP23 antibody (ab3340) + Rat brain tissue
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ICC/IF image of ab3340 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab3340 は 19 報の論文で使用されています。
- Mitani F et al. SNAP23-Mediated Perturbation of Cholesterol-Enriched Membrane Microdomain Promotes Extracellular Vesicle Production in Src-Activated Cancer Cells. Biol Pharm Bull 45:1572-1580 (2022). PubMed: 36184518
- Cardenas RA et al. SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis. J Biol Chem 296:100268 (2021). PubMed: 33837726
- Osawa Y et al. EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10:N/A (2021). PubMed: 33973520
- Chen F et al. ATG16L1 autophagy pathway regulates BAX protein levels and programmed cell death. J Biol Chem 295:15045-15053 (2020). PubMed: 32848017
- Yang Y et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761