Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (mammary gland tumors)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate antigen retrieval
Permeabilization
No
Specification
mammary gland tumors
Blocking step
10% goat serum in 1% BSA/PBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 27°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C · Diluent: 10% goat serum in 1% BSA/PBS
Dilution
1/200
Secondary antibody
Dilution
1/1500
Name
Non-Abcam antibody was used: biotin-goat anti-rabbit IgG, Vector Labs
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Additional data
Additional Notes
Canine fibrosarcoma was used as positive control.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 19 2016