Application
ChIP
Sample
Human Cell lysate - whole cell (DAOY and ONS76 Cells)
Specification
DAOY and ONS76 Cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step
Semiquantitative PCR
Positive control
Whole cell lysate
Negative control
Buffer Control
Other product details
Dilution
1/500
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: From Millipore Magnetic ChIP Kit
Additional data
Additional Notes
Did not pull down protein any more than mouse IgG control.
Abcam response
Note that this review is describing the use of ab78105 in an untested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 09 2009