Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Dilution
1/200
Incubation time
30 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
The antibody appears to robustly recognize an epitope in the nucleus. However, the antibody does not appear to recognize any chromatin-associated epitope (ie cohesin) during the initial stages of mitosis (prophase and prometaphase) and is perhaps due to epitope exclusion by increase chromatin compaction.
I also tested this antibody in Indian muntjac cells where it gave a similar staining pattern to that described above. (Attached is an Indian muntjac interphase cell labeled with anti-SMC3).
I also tested this antibody in Indian muntjac cells where it gave a similar staining pattern to that described above. (Attached is an Indian muntjac interphase cell labeled with anti-SMC3).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Dec 07 2005