Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
40 µg
Specification
HeLa cells
Treatment
X radiation (10g)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Other product details
Dilution
1/1500
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: DAKO P0217
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 150 kDa Non-specific: others kDa
Positive control
irradiated cells
Negative control
non irradiated cells
Additional data
Additional Notes
Figure legend : 1- non irradiated cells ; 2- 5' after irradiation 3- 15' after IR ; 4- 30' after IR ; 5- 45' after IR ; 6- 1h after IR and 7- 2h after IR
secondary antibodies host specie = swine
secondary antibodies host specie = swine
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 28 2007