Application
ChIP
Sample
Human Cell lysate - nuclear (MCF7 breast epithelial adenocarcinoma)
Specification
MCF7 breast epithelial adenocarcinoma
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
Region upstream of the transcription start site of the TFF1 gene
Region upstream of the transcription start site of the TK1 gene
Region upstream of the transcription start site of the TK1 gene
Negative control
Region on chromosome 12 (Untr12) that is far from any known gene annotation and not expected to be bound by BRM1
Other product details
Concentration
0.26 µg/µg chromatin
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SDS, DOC, TritonX-100, EDTA, HEPES, NaCl
Additional data
Additional Notes
Cells were treated with estrogen or vehicle (ethanol) as a control. Estrogen is expected to induce TFF1 and TK1 expression. The slight increase in BRM binding after estrogen treatment correlates with increased gene expression.
Ct values were converted to DNA copy numbers using a standard curve in the Q-PCR step. The number of binding events detected for each test reaction was then calculated by taking into account the DNA copy number, cell equivalents of chromatin used in the ChIP and PCR, and primer pair amplification efficiency.
Genpathway provides ChIP-Seq, ChIP-on-Chip and ChIP-QPCR services.
www.genpathway.com
Ct values were converted to DNA copy numbers using a standard curve in the Q-PCR step. The number of binding events detected for each test reaction was then calculated by taking into account the DNA copy number, cell equivalents of chromatin used in the ChIP and PCR, and primer pair amplification efficiency.
Genpathway provides ChIP-Seq, ChIP-on-Chip and ChIP-QPCR services.
www.genpathway.com
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Genpathway Inc
Verified customer
投稿 May 12 2009