Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8% gel)
Sample
Human Cell lysate - whole cell (Human Umbilical Artery Smooth Muscle Cells)
Specification
Human Umbilical Artery Smooth Muscle Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit Immunoglobulins
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL+
Bands
Specific: 22 kDa
Exposure
2 minute(s) and 0 second(s)
Negative control
Human Fibroblast, Human Umbilical Vein Endothelial Cells (HUVEC)
Positive control
Human Umbilical Artery Smooth Muscle Cells (UASMC)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 07 2013