Anti-TAGLN/Transgelin 抗体 (ab10135)
Key features and details
- Goat polyclonal to TAGLN/Transgelin
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, Flow Cyt
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TAGLN/Transgelin antibody
TAGLN/Transgelin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TAGLN/Transgelin -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Human
交差が予測される動物種: Cow, Dog -
免疫原
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ポジティブ・コントロール
- WB: Human testes lysate
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特記事項
In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22-kD protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus. The protein is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22-kD protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus. The protein is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10135の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
Primary incubation for 1 hour at room temperature.
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IHC-P |
Use at an assay dependent concentration. PubMed: 16080196
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 28955046
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ICC/IF |
Use at an assay dependent concentration. PubMed: 22257561
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 26183159
|
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 17099777
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa). Primary incubation for 1 hour at room temperature.
|
IHC-P
Use at an assay dependent concentration. PubMed: 16080196 |
IHC-Fr
Use at an assay dependent concentration. PubMed: 28955046 |
ICC/IF
Use at an assay dependent concentration. PubMed: 22257561 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 26183159 |
Flow Cyt
Use at an assay dependent concentration. PubMed: 17099777 ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. -
配列類似性
Belongs to the calponin family.
Contains 1 calponin-like repeat.
Contains 1 CH (calponin-homology) domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6876 Human
- Entrez Gene: 21345 Mouse
- Entrez Gene: 25123 Rat
- SwissProt: Q01995 Human
- SwissProt: P37804 Mouse
- SwissProt: P31232 Rat
- Unigene: 410977 Human
- Unigene: 283283 Mouse
see all -
別名
- 22 kDa actin-binding protein antibody
- Protein WS3-10 antibody
- SM22 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (125)
ab10135 は 125 報の論文で使用されています。
- Facchinello N et al. Oxidative pentose phosphate pathway controls vascular mural cell coverage by regulating extracellular matrix composition. Nat Metab 4:123-140 (2022). PubMed: 35102339
- Ciccimarra R et al. The normal and fibrotic mouse lung classified by spatial proteomic analysis. Sci Rep 12:8742 (2022). PubMed: 35610327
- Cao R et al. Molecular Mechanism of Mouse Uterine Smooth Muscle Regulation on Embryo Implantation. Int J Mol Sci 23:N/A (2022). PubMed: 36293350
- Liu SM et al. Intermedin Alleviates Vascular Calcification in CKD through Sirtuin 3-Mediated Inhibition of Mitochondrial Oxidative Stress. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 36297336
- Namiguchi K et al. Unique Angiogenesis From Cardiac Arterioles During Pericardial Adhesion Formation. Front Cardiovasc Med 8:761591 (2021). PubMed: 35187100