Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer tissue, tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6
Permeabilization
Yes - tween 20
Specification
breast cancer tissue, tonsil
Blocking step
ab diluent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: ab diluent
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested in IHC-P and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 07 2017