Application
Western blot
Sample
Human Cell lysate - whole cell (Hela Cells)
Loading amount
20 µg
Specification
Hela Cells
Gel Running Conditions
Non-reduced Denaturing (7.5% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween
Secondary antibody
Name
Non-Abcam antibody was used: Dako Poly Mouse Anti-Rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 145-150 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Jun 14 2011