製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC12A6
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human SLC12A6.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human tissue lysates: Kidney; Skeletal Muscle.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

ターゲット情報

  • 機能
    Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
  • 組織特異性
    Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
  • 関連疾患
    Agenesis of the corpus callosum, with peripheral neuropathy
  • 配列類似性
    Belongs to the SLC12A transporter family.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Basolateral cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACCPN antibody
    • Electroneutral potassium-chloride cotransporter 3 antibody
    • Furosemide sensitive KCl cotransporter 3 antibody
    • Gaxp antibody
    • K-Cl cotransporter 3 antibody
    • KCC 3 antibody
    • KCC 3A antibody
    • KCC 3B antibody
    • KCC3 A antibody
    • KCC3 antibody
    • KCC3 B antibody
    • KCC3A antibody
    • KCC3B antibody
    • Potassium chloride cotransporter 3 antibody
    • Potassium chloride cotransporter KCC3a S3 antibody
    • S12A6_HUMAN antibody
    • SLC12 A6 antibody
    • SLC12A 6 antibody
    • SLC12A6 antibody
    • Solute carrier family 12 (potassium/chloride transporters), member 6 antibody
    • Solute carrier family 12 member 6 antibody
    • Solute carrier family 12, member 6 antibody
    see all

画像

  • All lanes : Anti-SLC12A6 antibody (ab92951) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 128 kDa
    Observed band size : 122,128 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab92951 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92951 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa cells at 1ug/ml, and in Methanol fixed (100%, 5min) HepG2 cells at 1ug/ml

参考文献

ab92951 has not yet been referenced specifically in any publications.

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