Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing
Loading amount
500000 cells
Specification
melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Detection
Detection method
ECL
Negative control
None.
Exposure
15 second(s)
Bands
Specific: 39 kDa Non-specific: 35 kDa
Positive control
We tested 3 antibodies at the same time and observed one stronger band of the expected molecular weight of 39kDa (ab62739, ab176345, ab88494).
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投稿 Apr 23 2015