Application
Western blot
Sample
Rabbit Tissue lysate - other (Heart and placenta)
Gel Running Conditions
Non-reduced Denaturing (13% Acrylamide)
Loading amount
40 µg
Specification
Heart and placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk, Tween20 and Phosphate Buffer
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-goat
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminiscent
Exposure
1 minute(s) and 39 second(s)
Bands
Specific: 29 kDa Non-specific: 50, 15 kDa
Positive control
Mitochondria isolated from human placenta and human heart lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 15 2016