Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse fibroblast)
Loading amount
50 µg
Specification
mouse fibroblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBST
Secondary antibody
Name
Non-Abcam antibody was used: goat HRP-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 40 kDa
Additional data
Abcam response
We appreciate the valuable data provided in this Abreview. The testing of ab51023 in our laboratory showed no positive signal in mouse heart, brain and skeletal muscle where SIRT2 is highly expressed. Additionally, there is only 60% sequence homology with mouse SIRT2 (Uniprot ID: Q8VDQ8). As a result, this antibody was released as not reactive with mouse (or rat). We would appreciate to receive other customers experience regarding mouse as sample type with this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 07 2013