Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (oocyte)
Permeabilization
Yes - 3.0% TRITON X-100
Specification
oocyte
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5.0% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3.0-5.0%BAS in PBS(-)
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Mouse oocyte at germinal vesicle were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody weren't stained nucleus but cytoplasm.
Abcam response
The species (mouse) was not previously tested.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 13 2016