Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Cynomolgus Monkey Tissue lysate - whole (Peripheral Blood Mononuclear Cell)
Specification
Peripheral Blood Mononuclear Cell
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: 0.1 mg/mL Anti-Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/2000
Detection
Detection method
Super Signal West Femto
Bands
Specific: 55 kDa Non-specific: 100 kDa
Exposure
5 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Daniel Tyrrell
Verified customer
投稿 Sep 27 2013