Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Diluent: Blocker 1:1 in PBS + 0.1%Tween
Dilution
1/750
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
LI-COR® Odyssey® detection system
Bands
Specific: 120 kDa Non-specific: 47, 52, 350 kDa
Exposure
2 minute(s) and 0 second(s)
Negative control
-
Positive control
MEF whole cell extract
Additional data
Abcam response
Thank you very much for your feedback.
For users wishing to test our products in untested species or applications, we would encourage them to participate in our Abtrial program, by which you will beneficiate from a discount off your next purchase for the value of the product tested by submitting your results to our website.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
We would welcome more feedback from our customers in order to make further decision about the suitability of this antibody for Mouse.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 30 2013