Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Diluent: Blocker 1:1 in PBS + 0.1%Tween
Dilution
1/750
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
LI-COR® Odyssey® detection system
Bands
Specific: 120 kDa Non-specific: 12, 52, 58, 350 kDa
Exposure
2 minute(s) and 0 second(s)
Negative control
-
Positive control
HeLa whole cell extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 30 2013