Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse and human Embryonic stem cell lysate)
Loading amount
40 µg
Specification
mouse and human Embryonic stem cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBST
Secondary antibody
Name
Non-Abcam antibody was used: HRP goat anti-rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL (invitrogen)
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 130 kDa
Additional data
Additional Notes
mouse SIRT1 Theoretical Mw: 80.3 KD
human SIRT1 Theoretical Mw: 81.6 KD
We could detect mouse SIRT1 with this antibody
human SIRT1 Theoretical Mw: 81.6 KD
We could detect mouse SIRT1 with this antibody
Abcam response
We would like to thank this customer for their valuable feedback. After receiving this positive review with mouse samples, we tested mouse samples in our lab since the homology of the Immunogen is low with only 68%.
We could not detect a positive signal in our mouse samples (brain, heart, kidney, spleen) in WB.
We would like to get feedback from other users that have used this antibody with mouse samples.
We could not detect a positive signal in our mouse samples (brain, heart, kidney, spleen) in WB.
We would like to get feedback from other users that have used this antibody with mouse samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 03 2013