Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Cell lysate - other (Epithelial cells)
Specification
Epithelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
5 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/8000
Detection
Detection method
ECL
Bands
Specific: 120 kDa Non-specific: 90, 56, 24 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 21 2013