Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Jurkat
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Blocking buffer
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-Linked Whole Ab Sheep
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 56 kDa Non-specific: 47 kDa
Additional data
Additional Notes
The detail of 47 kDa band is unclear, but expected band at 56 kDa is reproducibly detectable.
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投稿 May 02 2012