Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
Sample
Human Cell (Human Renal Proximal Tubular Endothelial Cells)
Specification
Human Renal Proximal Tubular Endothelial Cells
Permeabilization
Yes - triton
Fixative
Paraformaldehyde
Other product details
Incubation time
21 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+donkey serum
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti mouse-FITC labelled
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/50
Additional data
Additional Notes
Some "stains" are detectable at this dilution (1/500). Maybe this antibody can be used at lower concentrations for IF on cultured cells.
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投稿 Mar 04 2015