Application
Western blot
Sample
Chicken Cell lysate - other (Chicken lymphoblasts DT40)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Chicken lymphoblasts DT40
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in PBST
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat Anti Rabbit IgG (Jackson)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Negative control
No negative samples were tested.
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Positive control
Recognize Flag-Tagged form of chicken SET.
Additional data
Additional Notes
Antibody is very clean and works well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Jul 19 2010