Application
Western blot
Sample
Pig Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
1.5 µg
Specification
heart
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/20000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 115 kDa
Positive control
Normal heart tissue
Additional data
Additional Notes
N= normal region of a MI heart
P= peri-infract region of a MI heart
MI= myocardial infract region of a MI heart
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 06 2016