Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole body)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
whole body
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+5%milk+0.01% tween20
Dilution
1/200
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Immobilon Western Chemiluminescent
Negative control
Zebrafish with morfolino for SECISBP2
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 13 kDa Non-specific: 3.5, 24, 31, 57, 90 kDa
Positive control
WT zebrafish extract
Additional data
Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback about the use of ab124840 in Zebrafish, a species we have not tested before.We welcome any further feedback and AbReviews from other researchers who have used this antibody in Zebrafish which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 23 2018