Application
Western blot
Sample
Human Cell lysate - whole cell (human osteosarcoma cells)
Loading amount
20 µg
Specification
human osteosarcoma cells
Treatment
siRNA transfected with control and two senp5 siRNA
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 2%BSA TBS-0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 87 kDa Non-specific: 140 kDa
Negative control
siRNA treated cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sonia Rocha
Verified customer
投稿 Jun 18 2009