The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human SEC61A peptide (ab22804).
Use a concentration of 3.75 µg/ml.
Use at an assay dependent concentration. PubMed: 19934005
Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.