Anti-SDHC 抗体 [EPR11035(B)] (ab155999)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11035(B)] to SDHC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SDHC antibody [EPR11035(B)]
SDHC 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11035(B)] to SDHC -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to residues in Human SDHC (UniProt Q99643).
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ポジティブ・コントロール
- THP-1, HeLa and fetal liver lysates; Human liver and lung carcinoma tissues; rat liver lysates; THP-1; Mouse brain lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11035(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155999の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000. Predicted molecular weight: 19 kDa.
For unpurified use at 1/10000 - 1/50000. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. |
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IHC-P |
1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/250 -1/500. |
特記事項 |
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WB
1/10000. Predicted molecular weight: 19 kDa. For unpurified use at 1/10000 - 1/50000. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. |
IHC-P
1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/250 -1/500. |
ターゲット情報
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機能
Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). -
パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle. -
関連疾患
Defects in SDHC are the cause of hereditary paragangliomas type 3 (PGL3) [MIM:605373]; also known as autosomal dominant non-chromaffin paragangliomas type 3. Non-chromaffin paragangliomas are usually benign, neural crest derived tumors of parasympathetic ganglia.
Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also known as Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. -
配列類似性
Belongs to the cytochrome b560 family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6391 Human
- Entrez Gene: 66052 Mouse
- Entrez Gene: 289217 Rat
- Omim: 602413 Human
- SwissProt: Q99643 Human
- SwissProt: Q9CZB0 Mouse
- Unigene: 444472 Human
- Unigene: 198138 Mouse
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別名
- CYB560 antibody
- C560_HUMAN antibody
- CYBL antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling SDHC with purified ab155999 at 1:700 dilution (0.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/10000 dilution (unpurified)
Lane 1 : THP-1 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa -
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SDHC with unpurified ab155999 at 1/250 dilution.
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Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/50000 dilution (purified) + Rat liver lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 19 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/10000 dilution (purified)
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 2 : Human fetal liver lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling SDHC with unpurified ab155999 at 1/250 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab155999 は 9 報の論文で使用されています。
- Li J et al. Comprehensive Analysis of Phagocytosis-Related Regulators to Aid Prognostic Prediction and Immunotherapy in Patients with Low-Grade Glioma. Dis Markers 2022:4142684 (2022). PubMed: 35510040
- Wang W et al. Dl-3-n-butylphthalide attenuates mouse behavioral deficits to chronic social defeat stress by regulating energy metabolism via AKT/CREB signaling pathway. Transl Psychiatry 10:49 (2020). PubMed: 32066705
- Anwar T et al. Next-generation sequencing identifies recurrent copy number variations in invasive breast carcinomas from Ghana. Mod Pathol 33:1537-1545 (2020). PubMed: 32152520
- Jiang C et al. Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1. Front Cell Dev Biol 8:603688 (2020). PubMed: 33409279
- Xie Y et al. Mefloquine Inhibits Esophageal Squamous Cell Carcinoma Tumor Growth by Inducing Mitochondrial Autophagy. Front Oncol 10:1217 (2020). PubMed: 32850358
- Bezawork-Geleta A et al. Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints. Nat Commun 9:2221 (2018). PubMed: 29880867
- Gómez-Serrano M et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). WB ; Human . PubMed: 28064117
- Haller F et al. Aberrant DNA hypermethylation of SDHC: a novel mechanism of tumor development in Carney triad. Endocr Relat Cancer 21:567-77 (2014). PubMed: 24859990
- Sverdlov AL et al. High fat, high sucrose diet causes cardiac mitochondrial dysfunction due in part to oxidative post-translational modification of mitochondrial complex II. J Mol Cell Cardiol N/A:N/A (2014). WB ; Mouse . PubMed: 25109264