Anti-SCRIBBLE 抗体 [EPR4140(2)] (ab125080)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4140(2)] to SCRIBBLE
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SCRIBBLE antibody [EPR4140(2)]
SCRIBBLE 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4140(2)] to SCRIBBLE -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide within Human SCRIBBLE aa 1550-1650 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- 293T, HeLa and MCF7 cell lysates; permeabilized 293T cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4140(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125080の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa).
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa). |
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. -
組織特異性
Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level). -
配列類似性
Belongs to the LAP (LRR and PDZ) protein family.
Contains 16 LRR (leucine-rich) repeats.
Contains 4 PDZ (DHR) domains. -
翻訳後修飾
Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded. -
細胞内局在
Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions. - Information by UniProt
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参照データベース
- Entrez Gene: 23513 Human
- Omim: 607733 Human
- SwissProt: Q14160 Human
- Unigene: 436329 Human
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別名
- CRIB 1 antibody
- CRIB1 antibody
- hScrib antibody
see all
画像
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All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SCRIB knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 175 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab125080 observed at 240 kDa. Red - loading control ab8245 observed at 36 kDa.
ab125080 Anti-SCRIBBLE antibody [EPR4140(2)] was shown to specifically react with SCRIBBLE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265190 (knockout cell lysate ab257660) was used. Wild-type and SCRIBBLE knockout samples were subjected to SDS-PAGE. ab125080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 175 kDa -
ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab125080 は論文での使用が確認できていません。