Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human fetal ovary)
Specification
human fetal ovary
Fixative
neutral buffered formalin
Permeabilization
No
Blocking step
5% BSA / 20% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/50000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA / 20% serum
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG + tyramide amplification (red
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200
Additional data
PubMed ID
22296229: View
Additional Notes
Red: anti-SCP3 (Abcam, ab15093), Green: anti-LIN28 (Abcam, ab46020).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 21 2012