Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Ovary)
Specification
Mouse Ovary
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris Buffer pH 10.0
Permeabilization
Yes - Tween20
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: DyLight 488 AffiniPure Donkey Anti-Rabbit IgG(H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight® 488
Dilution
1/500
Additional data
Abcam response
Previous batches of this antibody (such as the one used in this abreview) have cross-reacted with mouse. However, we have no evidence to show that the current batch cross-reacts with this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 06 2010