Application
Western blot
Sample
Human Cell lysate - whole cell (HuH7.5 Cell line ( hepatocyte-derived carcinoma ce)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Specification
HuH7.5 Cell line ( hepatocyte-derived carcinoma ce
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% BSA in PBS
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 140 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Apr 17 2017