Application
Western blot
Sample
Mouse Cell lysate - whole cell (Bone marrow cell)
Gel Running Conditions
Reduced Denaturing (Any kDMini-PROTEAN TGX Gel)
Loading amount
500000 cells
Specification
Bone marrow cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk in TNT
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: NA934V
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you for your valuable feedback and for participating in our Abtrial testing program. We are sorry to hear that this antibody has not given you good results in Western blot using mouse sample. This antibody has not been tested with this Western blot / mouse before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 10 2015