Anti-SART1 抗体 (ab95957)
Key features and details
- Rabbit polyclonal to SART1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SART1 antibody
SART1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SART1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment, corresponding to a region within amino acids 137 - 417 of Human SART1
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ポジティブ・コントロール
- A431,H1299, MOLT4 and Raji cells. Cal27 xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95957の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 90 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 90 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the SNU66/SART1 family. -
翻訳後修飾
Sumoylated with SUMO2. -
細胞内局在
Nucleus. Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells. - Information by UniProt
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参照データベース
- Entrez Gene: 9092 Human
- Entrez Gene: 20227 Mouse
- Entrez Gene: 29678 Rat
- Omim: 605941 Human
- SwissProt: O43290 Human
- SwissProt: Q9Z315 Mouse
- SwissProt: Q5XIW8 Rat
- Unigene: 502883 Human
see all -
別名
- Ara 1 antibody
- ARA1 antibody
- HOMS 1 antibody
see all
画像
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Anti-SART1 antibody (ab95957) at 1/5000 dilution + A431 whole cell lysate
at 30 µg
Predicted band size: 90 kDa -
ab95957, at a 1/200 dilution, staining SART1 in paraformaldehyde fixed A431 by Immunofluorescence.
Image on the right: merged with DNA probe. -
ab95957, at a 1/500 dilution, staining SART1 in paraffin embedded Cal27 xenograft by Immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab95957 は 2 報の論文で使用されています。
- Li Z et al. LncRNA FOXD3-AS1 Promotes Tumorigenesis of Glioma via Targeting miR-128-3p/SZRD1 Axis. Cancer Manag Res 13:9037-9048 (2021). PubMed: 34916848
- Ambrosetti D et al. The two glycolytic markers GLUT1 and MCT1 correlate with tumor grade and survival in clear-cell renal cell carcinoma. PLoS One 13:e0193477 (2018). PubMed: 29481555