Anti-SART1 抗体 (ab88583)
Key features and details
- Mouse polyclonal to SART1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SART1 antibody
SART1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to SART1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human SART1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- SART1 transfected 293T cell lysate, Jurkat cell lysate, HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88583の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the SNU66/SART1 family. -
翻訳後修飾
Sumoylated with SUMO2. -
細胞内局在
Nucleus. Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells. - Information by UniProt
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参照データベース
- Entrez Gene: 9092 Human
- Omim: 605941 Human
- SwissProt: O43290 Human
- Unigene: 502883 Human
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別名
- Ara 1 antibody
- ARA1 antibody
- HOMS 1 antibody
see all
画像
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Anti-SART1 antibody (ab88583) at 1 µg/ml + Jurkat cell lysate at 50 µg
Secondary
Goat anti-Mouse IgG (H+L) HRP at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
All lanes : Anti-SART1 antibody (ab88583) at 1 µg/ml
Lane 1 : SART1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution
Predicted band size: 90 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
ab88583 at 10µg/ml staining SART1 in HeLa cells by Immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab88583 は 3 報の論文で使用されています。
- Taylor SE et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Open Biol 6:N/A (2016). ICC/IF ; Human . PubMed: 27655733
- Laetsch TW et al. Multiple components of the spliceosome regulate Mcl1 activity in neuroblastoma. Cell Death Dis 5:e1072 (2014). WB . PubMed: 24556687
- Allen WL et al. A systems biology approach identifies SART1 as a novel determinant of both 5-fluorouracil and SN38 drug resistance in colorectal cancer. Mol Cancer Ther 11:119-31 (2012). WB ; Human . PubMed: 22027693