Anti-SARA 抗体 [EPR5020] (ab124875)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5020] to SARA
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SARA antibody [EPR5020]
SARA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5020] to SARA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human SARA aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- Huan heart, A673, HT-1080, BxPC-3, Jutkat, HeLa, and Human fetal brain lysates, Human kidney tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解離定数(KD 値)
KD = 3.10 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5020 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124875の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization. -
組織特異性
Ubiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes. -
配列類似性
Contains 1 FYVE-type zinc finger. -
ドメイン
The SMAD binding domain (SBD) interacts with the MH2 domains of SMAD2 or SMAD3.
The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P. -
細胞内局在
Cytoplasm. Early endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9372 Human
- Entrez Gene: 313477 Rat
- Omim: 603755 Human
- SwissProt: O95405 Human
- Unigene: 532345 Human
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別名
- CG15667 antibody
- hSARA antibody
- LD33044p antibody
see all
画像
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All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution
Lane 1 : Human heart tissue lysate
Lane 2 : A673 cell lysate
Lane 3 : HT-1080 cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 156 kDa -
All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 156 kDa -
Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab124875 は 5 報の論文で使用されています。
- Chen DQ et al. Combined melatonin and poricoic acid A inhibits renal fibrosis through modulating the interaction of Smad3 and ß-catenin pathway in AKI-to-CKD continuum. Ther Adv Chronic Dis 10:2040622319869116 (2019). PubMed: 31452866
- Wang JQ et al. Knockdown of microRNA-17-5p Enhances the Neuroprotective Effect of Act A/Smads Signal Loop After Ischemic Injury. Neurochem Res 44:1807-1817 (2019). PubMed: 31093905
- Jing C et al. MicroRNA-17/20a impedes migration and invasion via TGF-ß/ITGB6 pathway in esophageal squamous cell carcinoma. Am J Cancer Res 6:1549-62 (2016). PubMed: 27508097
- Correia AC et al. FGF2 inhibits endothelial-mesenchymal transition through microRNA-20a-mediated repression of canonical TGF-ß signaling. J Cell Sci 129:569-79 (2016). PubMed: 26729221
- Kurgonaite K et al. Essential role of endocytosis for interleukin-4-receptor-mediated JAK/STAT signalling. J Cell Sci 128:3781-95 (2015). PubMed: 26306492